CoolLED currently offers LED excitation at 20 different wavelengths + white ("WH1"). New LED wavelengths are always under development. Please check regularly to see the latest list.
Common Fluorophores
Fluorophore |
LED |
Fluorophore |
LED |
Fluorophore |
LED |
Acridine |
470 |
mCherry |
585* |
GFP |
490 |
Alexa 488 |
490 |
Cy3 |
530 |
Hoechst |
365 |
Alexa 488 |
470* |
Cy3.5 |
550 |
Indo-Cyanine Green |
770 |
Alexa 594 |
595 |
Cy5 |
635 |
Propidium Iodide |
530 |
Alexa 594 |
585* |
Cy7 |
700 |
RFP |
550 |
Alexa 660 |
660 |
DAPI |
365* |
Rhodamine |
530 |
Auramine |
470 |
DAPI |
400 |
Spectrum Green |
490 |
Calcofluor White |
365* |
Ethidium Bromide |
500 |
Texas Red |
565 |
Calcofluor White |
400 |
FITC |
470* |
TRITC |
530 |
CFP |
440 |
FITC |
490 |
YFP |
490 |
mCherry |
565 |
GFP |
470* |
YFP |
500* |
* CoolLED recommended wavelength for that fluorophore based on common filter sets
Common LED wavelengths for live cell and fixed cell applications.
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